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v450 conjugated rat anti mouse ly6c  (Bio-Rad)


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    Bio-Rad v450 conjugated rat anti mouse ly6c
    FIGURE 6. 4PD-mediated in vivo silencing of STAT3 and C/EBPb restores the efficacy of antitumor vaccines. (A) CT26 tumor–bearing (25 mm2) mice (n = 3) were injected once with 4PD loaded with BrUTP and STAT3 and/or C/EBPb shRNA. At 2, 24, 72, and 120 h postinjection, single-cell suspensions from the tumors were labeled with Abs against CD11b, Ly6G, <t>Ly6C,</t> F4/80, CD206 (to identify gMDSCs, mMDSCs, TAMs, and M1/M2 TAMs), and anti BrU Ab to identify the in vivo–transfected cells (n = 3 mice per group per time point). Data were derived from two independent experiments. The table shows the ANOVA p values comparing the effect of treatment on each population at each time point. (B) CT26 tumor–bearing mice (n = 5 per group) were treated i.v. with 4PD loaded with STAT3- and/or C/EBPb-specific shRNAs. Twenty-four hours after the last injection, T cells were magnetically sorted, CFSE labeled, and tested in MLTCs against CT26. (C, D and E) Starting 9 d after challenge, CT26 tumor–bearing mice were treated i.v. three times a week with PBS or with 4PD loaded with STAT3-specific shRNA, C/EBPb-specific shRNA, or with both shRNAs (20 mg per mouse). At 10 and 17 d after challenge, mice were vaccinated via electroporation with pcDNA3 (D) or with gp70 encoding pcDNA3 (E). Tumor growth was monitored. *p , 0.05 versus control.
    V450 Conjugated Rat Anti Mouse Ly6c, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v450+conjugated+rat+anti+mouse+ly6c/Rat+anti+Mouse+Ly-6C/pm28396317-70-34-61
    Average 93 stars, based on 45 article reviews
    v450 conjugated rat anti mouse ly6c - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells."

    Article Title: 4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1600833

    FIGURE 6. 4PD-mediated in vivo silencing of STAT3 and C/EBPb restores the efficacy of antitumor vaccines. (A) CT26 tumor–bearing (25 mm2) mice (n = 3) were injected once with 4PD loaded with BrUTP and STAT3 and/or C/EBPb shRNA. At 2, 24, 72, and 120 h postinjection, single-cell suspensions from the tumors were labeled with Abs against CD11b, Ly6G, Ly6C, F4/80, CD206 (to identify gMDSCs, mMDSCs, TAMs, and M1/M2 TAMs), and anti BrU Ab to identify the in vivo–transfected cells (n = 3 mice per group per time point). Data were derived from two independent experiments. The table shows the ANOVA p values comparing the effect of treatment on each population at each time point. (B) CT26 tumor–bearing mice (n = 5 per group) were treated i.v. with 4PD loaded with STAT3- and/or C/EBPb-specific shRNAs. Twenty-four hours after the last injection, T cells were magnetically sorted, CFSE labeled, and tested in MLTCs against CT26. (C, D and E) Starting 9 d after challenge, CT26 tumor–bearing mice were treated i.v. three times a week with PBS or with 4PD loaded with STAT3-specific shRNA, C/EBPb-specific shRNA, or with both shRNAs (20 mg per mouse). At 10 and 17 d after challenge, mice were vaccinated via electroporation with pcDNA3 (D) or with gp70 encoding pcDNA3 (E). Tumor growth was monitored. *p , 0.05 versus control.
    Figure Legend Snippet: FIGURE 6. 4PD-mediated in vivo silencing of STAT3 and C/EBPb restores the efficacy of antitumor vaccines. (A) CT26 tumor–bearing (25 mm2) mice (n = 3) were injected once with 4PD loaded with BrUTP and STAT3 and/or C/EBPb shRNA. At 2, 24, 72, and 120 h postinjection, single-cell suspensions from the tumors were labeled with Abs against CD11b, Ly6G, Ly6C, F4/80, CD206 (to identify gMDSCs, mMDSCs, TAMs, and M1/M2 TAMs), and anti BrU Ab to identify the in vivo–transfected cells (n = 3 mice per group per time point). Data were derived from two independent experiments. The table shows the ANOVA p values comparing the effect of treatment on each population at each time point. (B) CT26 tumor–bearing mice (n = 5 per group) were treated i.v. with 4PD loaded with STAT3- and/or C/EBPb-specific shRNAs. Twenty-four hours after the last injection, T cells were magnetically sorted, CFSE labeled, and tested in MLTCs against CT26. (C, D and E) Starting 9 d after challenge, CT26 tumor–bearing mice were treated i.v. three times a week with PBS or with 4PD loaded with STAT3-specific shRNA, C/EBPb-specific shRNA, or with both shRNAs (20 mg per mouse). At 10 and 17 d after challenge, mice were vaccinated via electroporation with pcDNA3 (D) or with gp70 encoding pcDNA3 (E). Tumor growth was monitored. *p , 0.05 versus control.

    Techniques Used: In Vivo, Vaccines, Injection, shRNA, Labeling, Transfection, Derivative Assay, Electroporation, Control

    Related Articles

    Flow Cytometry:

    Article Title: 4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.
    Article Snippet: .. The following Abs were used for flow cytometry analysis: allophycocyaninor Brilliant Violet (BV)711–conjugated rat anti-mouse CD11b (clone M1/70; BD), PerCp-Cy5.5–conjugated rat anti-mouse Ly6G and Ly6C (clone RB68C5; BioLegend), allophycocyanin-Cy7–conjugated rat anti-mouse Ly6G (clone 1-A8), V450-conjugated rat anti-mouse Ly6C (clone AL-21), PEconjugated rat anti-mouse CD124 (clone mIL4R-M1), PE-Cy7–conjugated rat anti-mouse F4/80 (clone BM8; all from BD), FITC-conjugated rat antimouse F4/80 (clone A3-1; AbD Serotec), BV650-conjugated rat anti-mouse CD206 (clone C068C2; BioLegend), PE-Cy7–conjugated hamster antimouse CD11c (clone HL3; BD), eFluor 450–conjugated rat anti-mouse by guest on A pril 13, 2017 http://w w w .jim m unol.org/ D ow nloaded from CD49b (clone DX5; eBioscience), PE-conjugated mouse anti-mouse I-A[d] (clone AMS-32.1), allophycocyanin-conjugated hamster anti-mouse CD80 (clone 16-10A1), FITC-conjugated rat anti-mouse CD86 (clone GL1), allophycocyanin-Cy7–conjugated rat anti-mouse CD4 (clone GK1.5), PEconjugated rat anti-mouse CD25 (clone 3C7), PerCP-conjugated hamster anti-mouse CD3 (clone 145-2C11; all from BD), PerCP–eFluor 710– conjugated rat anti-mouse CD3 (clone 17A2; eBioscience), allophycocyaninCy7–conjugated rat anti-mouse CD19 (clone ID3; BD), allophycocyanin rat anti-mouse Foxp3 (clone FJK-16s; eBioscience), Pacific Blue– or BV711conjugated rat anti-mouse CD8 (clone 53-6.7; BD), allophycocyaninconjugated mouse anti BrdU (clone Bu20a; eBioscience), FITC-conjugated mouse anti-human CD33 (clone HIM3-4), allophycocyanin-H7–conjugated mouse anti-human CD14 (clone MfP9), Pacific Blue–conjugated mouse anti-human CD11b (clone ICRF44; all from BD), allophycocyaninconjugated mouse anti-human IL-4Ra (clone 25463; R&D Systems), and BV711-conjugated mouse anti-human HLA-DR (clone L243; BioLegend). p-STAT6 AF647 Ab (clone J71-773.58.11; BD) was used with Phosflow Perm Buffer IV, as per the manufacturer’s instructions. .. Live/dead fixable dead cell stain (Invitrogen) or DAPI (Sigma-Aldrich) were used to exclude dead cells.

    Affinity Magnetic Separation:

    Article Title: 4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.
    Article Snippet: .. The following Abs were used for flow cytometry analysis: allophycocyaninor Brilliant Violet (BV)711–conjugated rat anti-mouse CD11b (clone M1/70; BD), PerCp-Cy5.5–conjugated rat anti-mouse Ly6G and Ly6C (clone RB68C5; BioLegend), allophycocyanin-Cy7–conjugated rat anti-mouse Ly6G (clone 1-A8), V450-conjugated rat anti-mouse Ly6C (clone AL-21), PEconjugated rat anti-mouse CD124 (clone mIL4R-M1), PE-Cy7–conjugated rat anti-mouse F4/80 (clone BM8; all from BD), FITC-conjugated rat antimouse F4/80 (clone A3-1; AbD Serotec), BV650-conjugated rat anti-mouse CD206 (clone C068C2; BioLegend), PE-Cy7–conjugated hamster antimouse CD11c (clone HL3; BD), eFluor 450–conjugated rat anti-mouse by guest on A pril 13, 2017 http://w w w .jim m unol.org/ D ow nloaded from CD49b (clone DX5; eBioscience), PE-conjugated mouse anti-mouse I-A[d] (clone AMS-32.1), allophycocyanin-conjugated hamster anti-mouse CD80 (clone 16-10A1), FITC-conjugated rat anti-mouse CD86 (clone GL1), allophycocyanin-Cy7–conjugated rat anti-mouse CD4 (clone GK1.5), PEconjugated rat anti-mouse CD25 (clone 3C7), PerCP-conjugated hamster anti-mouse CD3 (clone 145-2C11; all from BD), PerCP–eFluor 710– conjugated rat anti-mouse CD3 (clone 17A2; eBioscience), allophycocyaninCy7–conjugated rat anti-mouse CD19 (clone ID3; BD), allophycocyanin rat anti-mouse Foxp3 (clone FJK-16s; eBioscience), Pacific Blue– or BV711conjugated rat anti-mouse CD8 (clone 53-6.7; BD), allophycocyaninconjugated mouse anti BrdU (clone Bu20a; eBioscience), FITC-conjugated mouse anti-human CD33 (clone HIM3-4), allophycocyanin-H7–conjugated mouse anti-human CD14 (clone MfP9), Pacific Blue–conjugated mouse anti-human CD11b (clone ICRF44; all from BD), allophycocyaninconjugated mouse anti-human IL-4Ra (clone 25463; R&D Systems), and BV711-conjugated mouse anti-human HLA-DR (clone L243; BioLegend). p-STAT6 AF647 Ab (clone J71-773.58.11; BD) was used with Phosflow Perm Buffer IV, as per the manufacturer’s instructions. .. Live/dead fixable dead cell stain (Invitrogen) or DAPI (Sigma-Aldrich) were used to exclude dead cells.



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    FIGURE 6. 4PD-mediated in vivo silencing of STAT3 and C/EBPb restores the efficacy of antitumor vaccines. (A) CT26 tumor–bearing (25 mm2) mice (n = 3) were injected once with 4PD loaded with BrUTP and STAT3 and/or C/EBPb shRNA. At 2, 24, 72, and 120 h postinjection, single-cell suspensions from the tumors were labeled with Abs against CD11b, Ly6G, <t>Ly6C,</t> F4/80, CD206 (to identify gMDSCs, mMDSCs, TAMs, and M1/M2 TAMs), and anti BrU Ab to identify the in vivo–transfected cells (n = 3 mice per group per time point). Data were derived from two independent experiments. The table shows the ANOVA p values comparing the effect of treatment on each population at each time point. (B) CT26 tumor–bearing mice (n = 5 per group) were treated i.v. with 4PD loaded with STAT3- and/or C/EBPb-specific shRNAs. Twenty-four hours after the last injection, T cells were magnetically sorted, CFSE labeled, and tested in MLTCs against CT26. (C, D and E) Starting 9 d after challenge, CT26 tumor–bearing mice were treated i.v. three times a week with PBS or with 4PD loaded with STAT3-specific shRNA, C/EBPb-specific shRNA, or with both shRNAs (20 mg per mouse). At 10 and 17 d after challenge, mice were vaccinated via electroporation with pcDNA3 (D) or with gp70 encoding pcDNA3 (E). Tumor growth was monitored. *p , 0.05 versus control.
    V450 Conjugated Rat Anti Mouse Ly6c, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/v450+conjugated+rat+anti+mouse+ly6c/Rat+anti+Mouse+Ly-6C/pm28396317-70-34-61
    Average 93 stars, based on 1 article reviews
    v450 conjugated rat anti mouse ly6c - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 6. 4PD-mediated in vivo silencing of STAT3 and C/EBPb restores the efficacy of antitumor vaccines. (A) CT26 tumor–bearing (25 mm2) mice (n = 3) were injected once with 4PD loaded with BrUTP and STAT3 and/or C/EBPb shRNA. At 2, 24, 72, and 120 h postinjection, single-cell suspensions from the tumors were labeled with Abs against CD11b, Ly6G, Ly6C, F4/80, CD206 (to identify gMDSCs, mMDSCs, TAMs, and M1/M2 TAMs), and anti BrU Ab to identify the in vivo–transfected cells (n = 3 mice per group per time point). Data were derived from two independent experiments. The table shows the ANOVA p values comparing the effect of treatment on each population at each time point. (B) CT26 tumor–bearing mice (n = 5 per group) were treated i.v. with 4PD loaded with STAT3- and/or C/EBPb-specific shRNAs. Twenty-four hours after the last injection, T cells were magnetically sorted, CFSE labeled, and tested in MLTCs against CT26. (C, D and E) Starting 9 d after challenge, CT26 tumor–bearing mice were treated i.v. three times a week with PBS or with 4PD loaded with STAT3-specific shRNA, C/EBPb-specific shRNA, or with both shRNAs (20 mg per mouse). At 10 and 17 d after challenge, mice were vaccinated via electroporation with pcDNA3 (D) or with gp70 encoding pcDNA3 (E). Tumor growth was monitored. *p , 0.05 versus control.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: 4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

    doi: 10.4049/jimmunol.1600833

    Figure Lengend Snippet: FIGURE 6. 4PD-mediated in vivo silencing of STAT3 and C/EBPb restores the efficacy of antitumor vaccines. (A) CT26 tumor–bearing (25 mm2) mice (n = 3) were injected once with 4PD loaded with BrUTP and STAT3 and/or C/EBPb shRNA. At 2, 24, 72, and 120 h postinjection, single-cell suspensions from the tumors were labeled with Abs against CD11b, Ly6G, Ly6C, F4/80, CD206 (to identify gMDSCs, mMDSCs, TAMs, and M1/M2 TAMs), and anti BrU Ab to identify the in vivo–transfected cells (n = 3 mice per group per time point). Data were derived from two independent experiments. The table shows the ANOVA p values comparing the effect of treatment on each population at each time point. (B) CT26 tumor–bearing mice (n = 5 per group) were treated i.v. with 4PD loaded with STAT3- and/or C/EBPb-specific shRNAs. Twenty-four hours after the last injection, T cells were magnetically sorted, CFSE labeled, and tested in MLTCs against CT26. (C, D and E) Starting 9 d after challenge, CT26 tumor–bearing mice were treated i.v. three times a week with PBS or with 4PD loaded with STAT3-specific shRNA, C/EBPb-specific shRNA, or with both shRNAs (20 mg per mouse). At 10 and 17 d after challenge, mice were vaccinated via electroporation with pcDNA3 (D) or with gp70 encoding pcDNA3 (E). Tumor growth was monitored. *p , 0.05 versus control.

    Article Snippet: The following Abs were used for flow cytometry analysis: allophycocyaninor Brilliant Violet (BV)711–conjugated rat anti-mouse CD11b (clone M1/70; BD), PerCp-Cy5.5–conjugated rat anti-mouse Ly6G and Ly6C (clone RB68C5; BioLegend), allophycocyanin-Cy7–conjugated rat anti-mouse Ly6G (clone 1-A8), V450-conjugated rat anti-mouse Ly6C (clone AL-21), PEconjugated rat anti-mouse CD124 (clone mIL4R-M1), PE-Cy7–conjugated rat anti-mouse F4/80 (clone BM8; all from BD), FITC-conjugated rat antimouse F4/80 (clone A3-1; AbD Serotec), BV650-conjugated rat anti-mouse CD206 (clone C068C2; BioLegend), PE-Cy7–conjugated hamster antimouse CD11c (clone HL3; BD), eFluor 450–conjugated rat anti-mouse by guest on A pril 13, 2017 http://w w w .jim m unol.org/ D ow nloaded from CD49b (clone DX5; eBioscience), PE-conjugated mouse anti-mouse I-A[d] (clone AMS-32.1), allophycocyanin-conjugated hamster anti-mouse CD80 (clone 16-10A1), FITC-conjugated rat anti-mouse CD86 (clone GL1), allophycocyanin-Cy7–conjugated rat anti-mouse CD4 (clone GK1.5), PEconjugated rat anti-mouse CD25 (clone 3C7), PerCP-conjugated hamster anti-mouse CD3 (clone 145-2C11; all from BD), PerCP–eFluor 710– conjugated rat anti-mouse CD3 (clone 17A2; eBioscience), allophycocyaninCy7–conjugated rat anti-mouse CD19 (clone ID3; BD), allophycocyanin rat anti-mouse Foxp3 (clone FJK-16s; eBioscience), Pacific Blue– or BV711conjugated rat anti-mouse CD8 (clone 53-6.7; BD), allophycocyaninconjugated mouse anti BrdU (clone Bu20a; eBioscience), FITC-conjugated mouse anti-human CD33 (clone HIM3-4), allophycocyanin-H7–conjugated mouse anti-human CD14 (clone MfP9), Pacific Blue–conjugated mouse anti-human CD11b (clone ICRF44; all from BD), allophycocyaninconjugated mouse anti-human IL-4Ra (clone 25463; R&D Systems), and BV711-conjugated mouse anti-human HLA-DR (clone L243; BioLegend). p-STAT6 AF647 Ab (clone J71-773.58.11; BD) was used with Phosflow Perm Buffer IV, as per the manufacturer’s instructions.

    Techniques: In Vivo, Vaccines, Injection, shRNA, Labeling, Transfection, Derivative Assay, Electroporation, Control